Fig. 2

LRIG1 overexpression inhibits tumor regeneration in AR^−/lo PCa xenografts. a, b LRIG1 overexpression inhibits tumor regeneration and growth of AR^- Du145 (a; two independent experiments shown) and PPC-1 (b) cells. PCa cells infected with control (CTL) or LRIG1-expressing lentiviral vectors (MOI 5), and subcutaneously (s.c) injected (cell # indicated) in NOD/SCID mice. Tumors were harvested at the days indicated. Tumor incidence, endpoint tumor weight, and P values are presented on the right. The P-values for tumor incidence and weight were determined using χ² test and Student’s t-test, respectively. c Representative IHC images of LRIG1 (Sigma mAb, 1:100 dilution), Ki67 (Novus Biological, 1:500 dilution), and cleaved LAMIN A for apoptosis (Cell Signaling, 1:500 dilution) staining in endpoint tumors. In all, 4 μm serial sections were stained for the molecules indicated. Original magnifications were ×400 and a scale bar was indicated on one panel. d, e Quantification of Ki67⁺ (d) and LAMIN A⁺ (e) cells in endpoint tumors. Bars, mean ± SEM (by counting a total of 1000–1500 cells from 2 to 3 tumors from each model. *P < 0.05; **P < 0.01 (two-tailed unpaired Student’s t-test). *Source data for Fig. 2a, b, d, e are provided as a Source Data file.