Fig. 4

Transgenic expression of LRIG1 inhibits Myc-driven tumorigenesis. a Schematic of the ARR2PB-LRIG1 construct. Human LRIG1 cDNA (3,282 bp) was cloned into EcoRI/NheI sites of pPB197 vector. b PCR genotyping of transgenes LRIG1 (top) or c-Myc (below) using genomic DNA from mouse tails. c qPCR analysis of human LRIG1 mRNA in prostates from both WT and LRIG1-Tg mice. LAPC4 cell RNA was used as a positive control. Results are representative of three experiments. d WB of human LRIG1 protein expression in the prostates of 2- or 3-month-old animals using two different human-specific LRIG1 antibodies. Note that Sigma mAb detected two bands at 143 kDa and ~110 kDa whereas Cell Signaling pAb detected mainly the 143 kDa band. e Representative whole-mount images of the prostates from WT and LRIG1-Tg mice at 4 and 8 weeks, and 6 and 9 months (n = 3–9 for various age groups). f Prostate weights of WT and LRIG1-Tg mice at 4 and 8 weeks (the number of animals analyzed are indicated in the bars). Note that at 8 weeks, the LRIG1-Tg prostates were slightly smaller than WT prostates (P = 0.0127; Student’s t-test). g, h The LRIG1;Myc prostate is smaller than Hi-Myc prostate. Shown are representative images of microdissected prostates (g) and average weight of prostate lobes (h) from the indicated genotypes at 4 weeks old (bars represent mean ± S.D and n = 9, 6, 8, and 6 for the four genotypes). In h, the P-value (*P < 0.05) was determined by Student’s t-test. i Aperio Scanscope images of HE (left) and Ki67 (right) stained sections of a pair of Hi-Myc and LRIG1;Myc prostates. Scale bar, 100 μm. j, k Representative HE (h) and Ki67 (i) images (original magnifications and scale bars indicated) in the LP and VP sections of indicated genotypes. l Representative HE images of prostates lobes in 7-month-old Hi-Myc and dTg mice. Scale bar, 20 μm. m Summary of prostate pathologies in the indicated genetic mouse models. Differences in incidence between the Hi-Myc and LRIG1;Myc phenotypes were analyzed by χ² test. *Source data for Fig. 4c, d, f, h are provided as a Source Data file.