Fig. 1
From: Cryo-EM structure of the human MLL1 core complex bound to the nucleosome
Cryo-EM structure of the MLL1RWSAD-NCP complex. a Schematic domain architectures for the core components of the human MLL1 complex used in the cryo-EM study. b Immunoblot to detect H3K4 methylation in the in vitro histone methyltransferase assay. Antibodies indicated on right. The substrates were free recombinant histone H3 (left) and the NCP (right), respectively. Immunoblots for unmodified H3 as well as RbBP5 and ASH2L included as controls. c Cryo-EM 3D reconstruction of the MLL1RWSAD-NCP complex. The composite map of MLL1RWSAD-NCP was locally filtered to the estimated resolution. The subcomplexes, i.e., RbBP5-NCP and MLL1RWS-NCP, shown in dashed boxes. d Top (left) and front (right) views of the MLL1RWSAD-NCP structure. The S-adenosyl-l-homocysteine (SAH) was represented as a sphere (red) and the MLL1 core components shown in cartoon representation (RbBP5: cyan, WDR5: green, MLL1SET: slate, ASH2L: pink, and DPY30 dimer: cerulean and teal). Widom 601 DNA and four histones were colored as indicated on the bottom. Two black dashed squares highlighted the nucleosome contact points near SHL1.5 and SHL7 by MLL1RWSAD. Illustrations of the protein structure and cryo-EM maps used in all figures were generated with PyMOL (Delano Scientific, LLC) and Chimera67/ChimeraX70.
