Fig. 1: Amplification of aSyn aggregates from brain extracts.
From: Structural heterogeneity of α-synuclein fibrils amplified from patient brain extracts
a Immunoblotting of PMCA products with proteinase-K (PK) digestion. Similar PK-resistant signals were detected in PD samples (PD1-PD3), whereas nothing remained in the control sample (CT; brain extract from an individual, in which an α-synucleinopathy was excluded). An uncropped image of the blot is shown in Supplementary Fig. 1. b ThT-binding kinetics of recombinant aSyn with or without PMCA products as seeds. ThT-intensities for three PD samples (PD1-PD3) were averaged and used to derive error bars. c Relative ThT-fluorescence after seeding with different PMCA products. Error bars indicate standard deviation over three fluorescence measurements for each sample.
