Fig. 5: Pulse-chase NAIL-MS analysis of turnover s2C after 20 mM MMS exposure in WT BW25113 and E. coli ΔalkB knockout strain. | Nature Communications

Fig. 5: Pulse-chase NAIL-MS analysis of turnover s2C after 20 mM MMS exposure in WT BW25113 and E. coli ΔalkB knockout strain.

From: NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli

Fig. 5

a Concept of turnover s2C generation and its detection by NAIL-MS. b Abundance of [34S] turnover s2C in WT BW25113 during recovery after MMS stress (20 mM) and unstressed control. c Abundance of [34S] turnover s2C in the ΔalkB strain during recovery after MMS stress (20 mM) and unstressed control. All experiments are from n = 3 biol. replicates and error bars reflect standard deviation. p-values from student t-test (equal distribution, two-sided): *p < 0.05, **p < 0.01 and ***p < 0.001. Source data are provided as a Source Data file.

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