Fig. 5: T255 O-GlcNAcylation induces the mitochondrial translocation of PGK1.
From: O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth
a Cellular distribution of PGK1 as determined by immunostaining in the presence or absence of OGT overexpression. HT-29 cells were transfected with GFP-fused PGK1 WT or T255V construct. Cells were co-stained with MitoTracker red, a fluorescent marker for mitochondria. b, c Immunoblotting analysis of cellular distribution of PGK1 WT or T255V in the presence or absence of OGT overexpression. d Immunoblotting analysis of PDHK1 and PDH phosphorylation in mitochondria. Mitochondrial fractions were prepared and immunoblotted with indicated antibodies. Total cell lysates were used as a control. Error bars denote the means ± standard deviations (SD). Statistical analyses were performed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001). Source data are provided as a Source Date file.
