Fig. 7: T255 O-GlcNAcylation of PGK1 regulates glucose metabolism in cells.
From: O-GlcNAcylation of PGK1 coordinates glycolysis and TCA cycle to promote tumor growth
a Comparison of glucose uptake, lactate production, ATP levels, and the total energy charge (ADP/ATP ratio) in PGK1 WT or T255V rescue HT-29 cells in the presence or absence of OGT overexpression. b The extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in PGK1 WT or T255V rescue HT-29 cells in the presence of OGT overexpression. c–f Relative abundance of metabolites derived from glycolysis (c), TCA cycle (d), the pentose phosphate pathway (e), and the serine synthetic pathway (f) in PGK1 WT or T255V rescue HT-29 cells in the presence of OGT overexpression. Error bars denote the means ± standard deviations (SD). Statistical analyses were performed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test (*P < 0.05, **P < 0.01, ***P < 0.001). Source data are provided as a Source Date file.
