Fig. 7: Effects of CAP-H2 condensin depletion on senescence markers. | Nature Communications

Fig. 7: Effects of CAP-H2 condensin depletion on senescence markers.

From: Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization

Fig. 7: Effects of CAP-H2 condensin depletion on senescence markers.The alternative text for this image may have been generated using AI.

a Schematic procedure to investigate effects of CAP-H2 condensin KD on the maintenance of OIS. IMR90 cells were infected with retrovirus encoding H-RasV12 and cultured for 9 days to establish OIS (Methods). OIS cells were then infected with lentivirus encoding one of the three shRNA constructs (#1, #2, and #3) against CAP-H2 gene (NCAPH2) or carrying an empty vector (control) and harvested 3 days after the lentivirus infection. b–d Effect of CAP-H2 KD on maintenance of OIS. Cells prepared as in panel a were subjected to DAPI (b), SA-β-gal (c), and BrdU (d) staining. P-values were calculated by two-sided Student’s t-test, using biologically independent samples (n = 3, error bars represent the SD).

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