Fig. 1: The MAGIC pipeline for genome-wide mapping genotype–phenotype relationships.
From: Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping
Guide sequences for genome-scale activation (orange), interference (light blue), and deletion (magenta) were synthesized as arrayed oligos on DNA chip and cloned into the corresponding gRNA expression plasmids using Golden-Gate Assembly. The iMAGIC library was constructed by transforming the pooled plasmid libraries into the CRISPR-AID integrated yeast strain, and subject to growth enrichment under various conditions or high throughput screening. The enrichment and depletion of guide sequences were profiled using next-generation sequencing. The iMAGIC workflow can be iterated to better understand and engineer complex phenotypes.
