Fig. 5: Influence of activator binding on PARP1 catalytic activity and vice versa.
From: PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
a Catalytic profile and parameters for PARP1 activation by H2A and H2A.X nucleosomes (mean ± s.d., n = 2 independent experiments). b Effect of auto-PARylation on activator binding behaviour. BLI measurements were performed in the presence of 100 μM NAD+ (with exception of uppermost sample), with PARP1 and either no activator, naked DNA or H2A or H2A.X nucleosome. Probes were switched to analyte(activator)-free conditions after 300 s (dashed line). Arrowheads indicate approximate inflexion points, coinciding with observable dissociation, for H2A and H2A.X nucleosome in the presence of NAD+. Source data are provided as a Source Data file.
