Table 1 Data collection and refinement statistics for NCP assembled with H2A.X, γH2A.X or H2A.X-S139E.
From: PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
H2A.X | γH2A.X | H2A.X-S139E | |
|---|---|---|---|
Data collection | |||
Space group | P212121 | P212121 | P212121 |
Cell dimensions | |||
a, b, c (Å) | 105.8, 109.9, 180.4 | 102.9, 109.4, 181.5 | 108.0, 109.5, 183.4 |
α, β, γ (°) | 90, 90, 90 | 90, 90, 90 | 90, 90, 90 |
Resolution (Å) | 2.85–93.9 (2.85–3.00)a | 2.75–93.7 (2.75–2.90)a | 2.20–94.0 (2.20–2.32)a |
Rmerge (%) | 19.3 (121) | 19.3 (252) | 6.6 (113) |
Rpim (%) | 9.0 (65.5) | 5.9 (77.9) | 3.5 (73.5) |
I/σI | 5.2 (1.1) | 8.4 (1.1) | 11.6 (1.0) |
CC½ (%) | 99.5 (59.8) | 99.9 (45.7) | 99.5 (51.4) |
Completeness (%) | 99.9 (99.8) | 100 (100) | 98.6 (91.2) |
Redundancy | 5.5 (4.2) | 11.9 (11.3) | 5.4 (3.3) |
Refinement | |||
Resolution (Å) | 2.85–93.9 | 2.75–93.7 | 2.20–94.0 |
No. of reflections | 47,274 | 52,860 | 106,763 |
Rwork/Rfree (%) | 26.5/32.0 | 22.0/27.6 | 23.2/27.4 |
No. of atoms | 12,157 | 12,255 | 12,281 |
Protein | 6174 | 6169 | 6233 |
DNA | 5939 | 6029 | 5939 |
Solvent | 44 | 57 | 109 |
B-factors (Å2) | 98 | 95 | 74 |
Protein | 68 | 74 | 56 |
DNA | 129 | 117 | 93 |
Solvent | 61 | 81 | 61 |
R.m.s. deviations | |||
Bond lengths (Å) | 0.006 | 0.004 | 0.008 |
Bond angles (°) | 1.428 | 1.210 | 1.482 |
