Table 2 Data collection and refinement statistics for NCP assembled with H2A and either the same blunt-end 145 bp DNA fragment (NCP145) used for the H2A.X structure or the same sticky-end 147 bp DNA fragment (NCP147s) used for the γH2A.X structure.
From: PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome
NCP145 | NCP147s | |
|---|---|---|
Data collection | ||
Space group | P212121 | P212121 |
Cell dimensions | ||
a, b, c (Å) | 107.6, 109.7, 183.5 | 105.3, 109.7, 183.8 |
α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
Resolution (Å) | 1.99–76.8 (1.99–2.10)a | 2.25–94.2 (2.25–2.37)a |
Rmerge (%) | 6.8 (212) | 9.5 (194) |
Rpim (%) | 2.1 (65.2) | 3.0 (76.0) |
I/σI | 17.4 (1.3) | 12.2 (1.0) |
CC½ (%) | 99.7 (52.6) | 99.7 (61.6) |
Completeness (%) | 99.6 (97.9) | 99.7 (97.7) |
Redundancy | 12.2 (11.6) | 11.5 (7.9) |
Refinement | ||
Resolution (Å) | 1.99–76.8 | 2.25–94.2 |
No. of reflections | 145,312 | 99,086 |
Rwork/Rfree (%) | 23.6/26.3 | 23.9/29.2 |
No. of atoms | 12,178 | 12,227 |
Protein | 6,118 | 6,129 |
DNA | 5,939 | 6,029 |
Solvent | 121 | 69 |
B-factors (Å2) | 79 | 85 |
Protein | 53 | 63 |
DNA | 106 | 108 |
Solvent | 44 | 66 |
R.m.s. deviations | ||
Bond lengths (Å) | 0.008 | 0.007 |
Bond angles (°) | 1.474 | 1.496 |
