Fig. 4: Protein expression capacity is the major axis of variation in gene expression in intestine cells.
From: Chaperone biomarkers of lifespan and penetrance track the dosages of many other proteins
a Boxplots of the amount of different types of variation for Phsp-16.2::mCherry reporter and PEMR-1::EMR-1:GFP in intestine cells; boxplots are plotted as described in Fig. 2. Here, η2(γ) and η2(P) are combined and colored purple; η2(G) is yellow as described in Fig. 1. y axis is unitless η2. A two-tailed t-test between combined η2(γ) and η2(P) vs η2(G) produced p < 0.05; data are from three independent experiments measuring cells from thirty animals (cells from ten animals in each independent experiments). b 3-dimensional scatterplot of expression levels of LMN-1::BFP, HIS-72::GFP, and EMR-1::mCherry in intestine cells. Data are from all intestine cells measured in the field of view for each of ten animals in one experiment; additional experiments and 2D scatterplots are shown in Supplementary Fig. 11. c Scatterplot of Phsp-16.2::GFP and Pvit-2::mCherry expression at whole animal level from three independent experiments. d Scatterplot of Peft-3::mTagBFP2 and Pvit-2::mCherry expression at whole animal level compiled from three independent experiments. In (d) and (e), r values shown are Pearson’s correlation coefficients and Ns are listed in each panel. Panel (e) shows images of animals from individual experiments quantifying Pmtl-2::GFP and Pdaf-21::mCherry (top panel), or, Peft-3::BFP and Pvit-2::mCherry (bottom panel). Animals are arranged from dimmest (left) to brightest (right); white scale bar on bottom right of top panel is 50 μm. Panel (f) shows a scatterplot of average expression levels for cells in each ring of individual animals from the bottom row of panel (e). Different animals are represented by shapes (circle (brightest), triangle (median), square (dimmest)). Different sized shapes indicate the ring of cells each is representing; ring one cells are the smallest, ring two cells are the medium sized, and ring three cells are the biggest. Panel (g) shows a scatterplot of all cells from all animals from all Type I (120 animals; white dots) and II experiments (480 animals; black dots), normalized and scaled.
