Fig. 5: HDAC3 and LH affected Areg expression by regulating SP1 recruitment to the Areg promoter.
From: HDAC3 maintains oocyte meiosis arrest by repressing amphiregulin expression before the LH surge
a Inhibition of SP1 reversed the HDACi 4b-mediated induction of Areg expression. MIT, SP1 inhibitor. Data are presented as mean ± SEM, and different letters (a–b) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.001. b Western blotting analysis revealed that inhibition of HDAC3 did not affect SP1 expression. HDACi 4b, HDAC3 inhibitor. n = 3 biologically independent experiments. Data are presented as mean ± SEM. c ChIP-qPCR analysis of SP1 at the Areg promoter (−343 to −150) in control and HDACi 4b-treated GCs. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.05, P (a, c) < 0.001, P (b, c) < 0.001. d SP1 levels in Hdac3flox/flox and Hdac3cKO GCs were quantified by western blotting analysis. n = 3 biologically independent experiments. Data are presented as mean ± SEM. Asterisks (*) indicate significant differences at ***P < 0.001 determined by the two-sided t test. e ChIP-qPCR analysis of SP1 at the Areg promoter in Hdac3flox/flox and Hdac3cKO GCs. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.05, P (a, c) < 0.001, P (b, c) < 0.001. f Inhibition of SP1 reversed the LH-mediated induction of Areg expression. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.001, P (a, c) < 0.001, P (b, c) < 0.001. g Western blotting analysis revealed that LH did not affect the SP1 expression in vivo. n = 3 biologically independent experiments. Data are presented as mean ± SEM. h ChIP-qPCR analysis of SP1 at the Areg promoter before and after the LH surge. P48: 48 h after PMSG treatment. H1–H4: 1–4 h after hCG treatment. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.05, P (a, c) < 0.001, P (b, c) < 0.001. Source data are provided as a Source Data file.
