Fig. 1: Colonic window with ferromagnetic scaffold for stabilized colon imaging.

a Diagram of the titanium colonic window with magnets placed on the surface above the ferromagnetic scaffold (green), located beneath the colon (red). The magnetic field (yellow) pulls the scaffold against the colon (arrows), holding the colon against the window during imaging. b Implantable ferromagnetic scaffold used to reversibly prevent colon movement during imaging. c Representative sum of 150 consecutive frames of vasculature (red) on the colon surface in a C57BL6/J mouse with and without the ferromagnetic scaffold activated by external magnets. d Quantification of tissue movement during time-series recordings of the colonic vasculature with and without the ferromagnetic scaffold activated by external magnets. Circles represent individual mice, and bars indicate μ ± s.e.m. Star indicates a statistically significant difference by unpaired, two-tailed t-test (n = 5, p < 0.05). e Mouse implanted with the colonic window. f Colonic window implanted in a mouse for up to 13 days after implantation. Arrows indicate tattoo mark on the colonic wall to track position within the colon. All scale bars are 100 μm.