Fig. 2: In vivo imaging of luminal content via the colonic window. | Nature Communications

Fig. 2: In vivo imaging of luminal content via the colonic window.

From: An intravital window to image the colon in real time

Fig. 2: In vivo imaging of luminal content via the colonic window.

a Representative images from a C57BL6/J mouse gavaged with adherent invasive E. coli derived from a patient with Crohn’s Disease and stably transformed to express GFP. Images taken from negative control, as well as 12 and 36 h after gavage of E. coli (green). Colonic crypts are visualized using second harmonic generation microscopy (blue). b Representative images from a Wnt1-tdTomato (red) mouse gavaged with Oregon-Green dye (green) and second harmonic generation microscopy (blue). c Quantification of Oregon-Green dye (OGD) fluorescence normalized to each subjects’ pre-gavage (0 h) timepoint. Circles represent individual mice by color, and bars indicate μ ± s.e.m. Star indicates statistically significant difference from control by Dunnett’s test for multiple comparisons after repeated-measures ANOVA (n = 3, p < 0.05). All scale bars are 100 μm.

Back to article page