Fig. 7: ZapA binds only transiently to FtsZ filaments. | Nature Communications

Fig. 7: ZapA binds only transiently to FtsZ filaments.

From: Cooperative ordering of treadmilling filaments in cytoskeletal networks of FtsZ and its crosslinker ZapA

Fig. 7

a Dual-color TIRF micrograph showing co-localization of Alexa488-FtsZ (cyan) and ZapA-Cy5 (magenta). b Raw image of FtsZ filament network overlaid with growing trajectories of FtsZ (cyan) and ZapA (magenta) from differential imaging (scale bar 5 μm). Kymographs are obtained along the orange arrow, which indicates treadmilling direction. Scale bars, t = 20 s, x = 3 μm. c Snapshots showing FRAP experiments of Cy5-labelled ZapA pattern. d Half-time recovery of Cy5-labelled ZapA showed a much faster recovery (ZapA = 3.01 ± 0.47, n = 6) when compared with FtsZ (Fig. 6b). e In contrast with FtsZ (Fig. 4d), the lifetime of ZapA was much shorter (ZapA = 0.95 ± 0.14, n = 5). Source data available at Figshare [https://doi.org/10.6084/m9.figshare.10347062.v1].

Back to article page