Fig. 1: Recombinant Abo1 is an ATPase that binds histone H3–H4.
From: Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone
a Conserved domain organization of S. pombe Abo1 and human ATAD2. We term the region between the AAA2 domain and bromodomain (shown in gray) the “linker arm” based on structural data shown below (Fig. 3b, c). b Gel filtration profile of Abo1 over a Superose6 column under different buffer (2 mM EDTA, 2 mM MgCl2, and 2 mM MgATP) conditions. c Binding of Abo1 to Cy3-labeled H3–H4 measured by fluorescence anisotropy assays. The Kd of Abo1 is 23 ± 13 nM. Error bars represent the standard error of the mean (SEM) for three experiments with different preparations of protein. d Steady-state ATPase rate of Abo1 in the presence of histone substrates. Error bars represent SEM for three experiments with different preparations of protein.
