Fig. 1: Characterization of the new 6hTau and T44mTauKO Tg mice.

a Comparison of total tau expression levels among the hT-PAC-N, 6hTau, WT, and T44mTauKO mouse lines, as well as human brain tau. Homogenates from cortices of the different mouse lines at the age of around 3 months or from normal human brains were probed with pan tau antibody K9JA, Mtau selective antibody T49, and Htau selective antibody T14. GAPDH served as a loading control. b Quantification of the relative total tau expression levels among the different brains as shown in a, n = 3 for each group. c Tau isoform expression pattern among brains of the different lines. Tau in brain homogenates were dephosphorylated with lambda protein phosphatase and probed, respectively, with antibodies K9JA and Tau5, 4R isoform tau-specific polyclonal antibody 4R Tau and 3R isoform tau-specific Mab RD3. The recombinant (re) six human tau isoforms were, respectively, loaded as standards on the right lanes. 6hTau mouse line express only human six tau isoforms, while T44mTauKO mouse line express only the shortest human 3R tau isoform, 0N3R. Loading control GAPDH were probed using same amounts of lysates without dephosphorylation. d Quantification of different tau isoform expression pattern in each mouse line. e developmental expression pattern of total tau and isoforms in WT and 6hTau mice at indicated ages. rh6hTau, recombinant six human tau isoforms. Quantification of the relative total tau expression levels in f, WT and g, 6hTau mice. Different isoform expression patterns during developmental stages in h WT and i 6hTau mice. j Blot images and k Quantifications for brain regional expression patterns of total tau among WT, 6hTau, T44, T44mTauKO mouse lines, and normal human brains were probed by tau antibody K9JA. GAPDH served as a loading control. l immunoblot images and quantifications of tau isoform expression patterns in different brain regions in m adult 6hTau mice and n normal human controls. OB olfactory bulb, Hpx hippocampus, Ctx cortex, CB cerebellum, BS brainstem, SC spinal cord, Fron frontal cortex, Temp temporal cortex, Occi occipital cortex, Puta putamen. Quantifications are presented as mean ± s.e.m., with each dot representing an individual. Source data is available as a Source Data file.