Fig. 4: VAB-10a/spectraplakin localization is disrupted in tbc-10;unc-70 mutants.

a, b Deconvolved spinning disk confocal maximum projection of typical endogenous VAB-10a::GFP localization at the L4 (a) and 1-day-old adult (1DOA) (b) stage in wild-type animals from a lateral perspective, with the PLM neuron labeled with a cytosolic TagRFP (Pmec-17::tagRFP, magenta). The side panels in a–d show the boxed area indicated in the left panel as a merged image and individual channels. c, d Deconvolved spinning disk confocal maximum projection of typical endogenous VAB-10a::GFP localization at the L4 (c) and 1DOA (d) stage in tbc-10(vd31);unc-70(n493) animals from a lateral perspective, with the PLM neuron labeled with a cytosolic TagRFP (Pmec-17::tagRFP, magenta). Arrow in d indicates a buckle in the PLM axon. Scale bars in a are 25 μm and 5 μm in the left and side panels, respectively. e Normalized intensities of VAB-10a::GFP along a 50 μm of the PLM axon at the L4 stage were scored as containing gaps (defined as >5 puncta with intensity values < 0.2 a.u.) or continuous attachment (defined as < 5 puncta with intensity values < 0.2 a.u.). f VAB-10a::GFP localization at the 1DOA stage was scored as continuous (no gaps in localization greater than 10 μm) or containing gaps (a region of no localization greater than 10 μm) with and without axonal breakage. N-values are indicated on charts and represent the number of individual animals scored for each condition.