Fig. 6: Attachment defects and axonal breakage are movement dependent. | Nature Communications

Fig. 6: Attachment defects and axonal breakage are movement dependent.

From: Epidermal control of axonal attachment via β-spectrin and the GTPase-activating protein TBC-10 prevents axonal degeneration

Fig. 6

a Mean penetrance of axonal breaks in tbc-10(vd31);vdSi2(Pdpy-7::mKate2::unc-70(n493)) animals with and without mutations in unc-54, acy-1, and egl-4. b Deconvolved spinning disk confocal maximum projection of typical endogenous VAB-10a::GFP localization in 1-day-old adult (1DOA) unc-54;tbc-10;vdSi2 animals from a lateral perspective, with the PLM neuron labeled with a cytosolic TagRFP (Pmec-17::tagRFP; magenta). The side panels in b show the boxed area indicated in the left panel as a merged image and individual channels. Scale bars in b are 25 μm and 5 μm in the left and side panels, respectively. c VAB-10a::GFP localization at the 1DOA stage was scored as continuous (no gaps in localization greater than 10 μm) or containing gaps (a region of no localization greater than 10 μm) with and without axonal breakage. P-values in a are determined from a one-way ANOVA with a Tukey multiple-comparison of proportions using an Agresti–Coull interval. Error bars indicate a 95% confidence interval. N-values are indicated on bar and pie charts and represent the number of individual animals scored for each condition. Source data are provided as a Source Data file.

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