Fig. 5: Temperature monitoring in vivo using UCL-NCs.

a Schematic diagram of surficial and intratumoral monitoring in vivo. b The standard curve for temperature evaluation in vivo measured with the same wavelength ratiometric probe based on UCL-NCs with the excitation of 865 nm laser using a home-built time-gated bioimaging system. c Real-time, time-gated and ratio UCL imaging without or with a 2 mm-pork tissue slice covering in vivo under irradiation by a 865 nm laser (0.5 W cm−2) for 30 s. The ratio imaging figure was obtained using image arithmetic (Ratio = (Real time − Time gated)/Time gated). Source data are provided as a Source Data file.