Fig. 4: Singleton TVRs enriched or depleted in ATRX/DAXX^trunc samples.

a Receiver operating characteristic for the classification of samples with ALT-associated mutations from telomere variant repeats. Red: no specific sequence context required. Blue: singletons ((TTAGGG)₃-NNNGGG-(TTAGGG)₃). The more the area under the curve (AUC) deviates from 0.5, the better the repeat occurrence distinguishes ATRX/DAXX^trunc from TERT^mod samples. b Pattern count tumor/control log2 ratios of all patients plotted against telomere content tumor/control log2 ratios for selected singletons. The regression line through the TERT^mod samples is shown in green and is defined as the expected pattern count in the following. c Distance to the expected singleton repeat count in ATRX/DAXX^trunc and TERT^mod samples. The center line of the boxplot is the median, the bounds of the box represent the first and third quartiles, the upper and lower whiskers extend from the hinge to the largest or smallest value, respectively, no further than 1.5 × IQR from the hinge (where IQR is the interquartile range, or distance between the first and third quartiles). ****p < 0.0001; ***p < 0.001, Wilcoxon rank-sum tests after Bonferroni correction. The profiles of all analyzed patterns are shown in Supplementary Figs. 11 and 13.