Fig. 2: Altered intratumoral effector T cells and Tregs in  Siah2^−/− mice.

a, b Quantification of tumor-infiltrating immune cells (CD45.2⁺), CD4⁺ and CD8⁺ T cells, CD11b⁺/F4/80⁺, and CD11c⁺ cells on day 11 after YUMMER1.7 cell injection. Quantification is reported as total number/g tumor a, and as percentage b of indicated immune cells among total cells (for the graph in b analyzing frequency of CD45.2⁺), or CD45.2⁺ cells (for other graphs in b; n = 4 for WT; n = 5 for KO). c, d Percentage of tumor-infiltrating T-bet⁺ c and FOXP3⁺ d cells within the CD4⁺ T cell population at day 11 after tumor inoculation (n = 5 for WT; n = 6 for Siah2^−/−). e–h Frequencies of tumor-infiltrating IFN-γ- and granzyme B-expressing CD4⁺ e, f or CD8⁺ g, h T cells 11 days after tumor cell inoculation and following stimulation overnight in vitro with melanoma peptides (n = 6) e–h; mean ± s.e.m. Data were analyzed by unpaired t-test. ***P < 0.0005, **P < 0.005, and *P < 0.05 compared with WT.