Table 2 Glutaminase activation of hsNadE and tbNadE.
From: Different ways to transport ammonia in human and Mycobacterium tuberculosis NAD+ synthetases
Enzyme | Effectors | Sample reactions | kcat (s−1) | Fold activation |
|---|---|---|---|---|
hsNadE | None | Glutamine | 0.180 ± 0.01 | -- |
NaAD+, ATP/Mg2+ | Glutamine | 5.6 ± 0.1 | 31 | |
NaAD+ | Glutamine | 0.316 ± 0.001 | 1.8 | |
NAD+ | Glutamine | 0.214 ± 0.002 | 1.2 | |
ATP/Mg2+ | Glutamine | 0.091 ± 0.002 | No activation | |
PPi/Mg2+ | Glutamine | 0.185 ± 0.002 | No activation | |
NAD+, ATP/Mg2+ | Glutamine | 0.113 ± 0.006 | No activation | |
NaAD+, AMP/Mg2+ | Glutamine | 0.375 ± 0.001 | 2.1 | |
NaAD+, AMP,PPi/Mg2+ | Glutamine | 2.2 ± 0.4 | 12 | |
tbNadE | None | Glutamine | 0.0038 ± 0.0002 | – |
NaAD+, ATP/Mg2+ | Glutamine | 0.68 ± 0.03 | 179a | |
NaAD+, AMP,PPi/Mg2+ | Glutamine | 0.167 ± 0.007 | 44b | |
SFI | Glutamine | 0.0021 ± 0.0002 | No activation | |
SFI, PPi/Mg2+ | Glutamine | 0.061 ± 0.004 | 16 |
