Fig. 5: Actin/myosin inhibition significantly reduces tearing off surface-bound particles.
From: Forces during cellular uptake of viruses and nanoparticles at the ventral side
a Inverted wide-field fluorescence images of HeLa cells fixed 90 min post drug addition. Actin filaments were immunolabeled with phalloidin-TRITC. Scale bar = 10 µm. b Projected cell area normalized to t0 of cells spreading on the matrix-mimetic surfaces decorated with nonspecific AuNPs (#1, see Fig. 4) over time. Actomyosin disrupting drugs Cytochalasin D (CytoD, 60 nM), Latrunculin A (LatA, 100 nM) or ROCK inhibitor (Y27632, 20 µM) were added 1 h after seeding of the cells. c Relative number of particles in the ROI under the cells over time after drug treatment. (n = 19, 15, 14, 15 cells, 2 technical repeats, data are represented as mean ± s.e.m. and fitted with an exponential decay function) d For the particles being actively removed by the HeLa cells, the fraction a and the off rate koff are shown as obtained by fitting from the two-phase-decay of particles in the ROI. (Box-plots represent median ± 95% CI with whiskers to the min and max values, *P = 0.0145, ****P < 0.0001; one-way ANOVA with post hoc Tukey). Source data are provided as a source data file.
