Fig. 2: Exposure to 5FC generates an adaptive advantage for mutator strains. | Nature Communications

Fig. 2: Exposure to 5FC generates an adaptive advantage for mutator strains.

From: 5-fluorocytosine resistance is associated with hypermutation and alterations in capsule biosynthesis in Cryptococcus

Fig. 2

Competition experiments between a tester strain with a neomycin resistance marker and a wildtype R265 strain. (Strains used: SEC501, RBB17, RBB18). Overnight cultures were mixed 1:1 and then used to inoculate a second overnight culture in liquid YNB with and without 5FC. All three marked strains showed a slight growth defect in comparison to the unmarked strain in nonselective media but only the hypermutator strains demonstrated a dramatic growth advantage when grown in YNB + 5FC. Boxplots show minimum, first quartile, median, third quartile, and maximum values. Points represent the results from three individual replicates and are summarized by the box plot. The R265 NEOR vs wildtype competition is gray, while the two independent msh2Δ::NEO vs wildtype competitions are dark and light blue.

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