Fig. 5: Confocal and STED time-lapse imaging of vimentin-emiRFP703.
From: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
a, b A confocal image series (a) and c, d a STED image series (c) of live HeLa cells transfected with emiRFP703-vimentin. The graphs (b, d) show the normalized integrated intensity of the recorded frames (red circles) for both image series, plotted every 40th frame for the confocal for better visualization (b) and every frame for STED (d). The displayed intensity scale has been adjusted per frame from zero to the maximum in respective frame. Scale bars, 2 µm.
