Table 1 Spectral and biochemical properties of the currently available BphP-derived monomeric NIR FPs.

From: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

Monomeric NIR FP

Parental BphP

Ex, nm

Em, nm

Extinction coefficient, M−1cm−1

Quantum yield, %

Molecular brightness vs. miRFP720, %a

Photostability in mammalian cells, t1/2, s

pKa

Eff. brightness in HeLa cells vs. miRFP720, %b

Reference

miRFP670

RpBphP1

642

670

87,400

14.0

204.7

490

4.5

67.9

16

miRFP670–2

RpBphP6

643

670

103,000

13.6

234.3

310

4.5

80.8

This paper

emiRFP670*c

RpBphP1

642

670

87,400

14.0

204.7

450

4.5

117.0

 

miRFP680*

RpBphP2

661

680

94,000

14.5

228.0

980

4.5

156.7

 

miRFP682

RpBphP2

663

682

91,000

11.2

170.5

500

4.5

101.8

 

miRFP702

RpBphP6

673

702

88,000

8.1

119.2

640

4.5

40.6

 

miRFP703

RpBphP1

674

703

90,900

8.6

130.8

650

4.5

49.8

16

emiRFP703*

RpBphP1

674

703

90,900

8.6

130.8

700

4.5

77.6

This paper

mIFP

BrBphP

683

705

65,900

6.9

76.1

90

4.5

13.9

19

miRFP709

RpBphP1

683

709

78,400

5.4

79.8

500

4.5

29.0

16

miRFP713*

RpBphP2

690

713

99,000

7.0

115.9

980

4.5

94.9

This paper

SNIFP

DrBphP

697

720

149,000

2.2

54.8

n.a.

4.5

n.a.

33

miRFP720*

RpBphP2

702

720

98,000

6.1

100.0

510

4.5

100

26

  1. aMolecular brightness is a product of extinction coefficient and fluorescence emission quantum yield
  2. bDetermined as a mean NIR fluorescence intensity of HeLa cells 48 h after transfection with no supply of exogenous BV
  3. cA set of five spectrally distinct monomeric NIR FPs, which is recommended for further use in imaging applications, is marked with asterisks
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