Fig. 7: Adipose OGT suppresses lipolysis and promotes diet-induced obesity and insulin resistance.
From: O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity
a Breeding strategy used to generate WT control mice and OGT AKI mice. b Western blot analysis of OGT and β-actin in eWAT from WT and OGT AKI mice. c Body weight of WT and OGT AKI mice fed on HFD (n = 11 WT, 10 KI). d–f Fat mass and lean mass of WT and OGT AKI mice fed on HFD for 4 weeks (n = 8 WT, 7 KI). g, h Images and tissue weights of fat pads from HFD-fed WT and OGT AKI mice (n = 4 WT, 6 KO for h). i IP and Western blot analysis showing the effects of OGT knockin on endogenous PLIN1 O-GlcNAcylation and phosphorylation; hollow arrowheads indicate IgG heavy chains. j Basal and stimulated (10 μM CL-316,243) lipolysis measured by glycerol released from explants of eWAT and iWAT from HFD-fed WT and OGT AKI mice (n = 2/group, three experiments). k–n Blood glucose and area under curve (AUC) analyses of glucose tolerance test and insulin tolerance test of HFD-fed WT and OGT AKI mice (n = 7 WT, 4 KI). o Molecular model for OGT function in lipolysis. Data are presented as mean ± s.e.m. Statistical analysis: Student’s t test, *p < 0.05, **p < 0.01, and ***p < 0.001. Source data are provided as a Source Data file.
