Fig. 2: Exo70 interacts with ULK1.
From: ULK1 phosphorylates Exo70 to suppress breast cancer metastasis
a Interaction of Exo70 and ULK1 in 293T cells was detected by co-immunoprecipitation assay. Whole-cell lysates (WCLs) and immunoprecipitated (IP) proteins were analyzed by western blotting. b The interaction of endogenous Exo70 with ULK1 in MCF-7 and MDA-MB-231 cells were examined by co-immunoprecipitation using anti-Exo70 or anti-IgG (negative control) antibody. c The interaction of Exo70 and ULK1 was determined by GST pull-down assay. Purified HA-ULK1 protein was incubated with recombinant GST-Exo70 protein conjugated to the Glutathione Sepharose. GST-Exo70 was examined by Coomassie Blue staining (lower panel) and the bounded HA-ULK1 was detected with anti-HA antibody (upper panel). GST was used as a negative control. d The co-localization of endogenous Exo70, ULK1, and cortactin was demonstrated with immunofluorescence in MCF-7 and MDA-MB-231 cells. Scale bar: 10 μm.
