Fig. 6: Complement mediated enhancement of Arenacept.

a Complement augments direct neutralization of Arenacept-M3. Neutralization of JUNV- and MACV-pseudotyped viruses was tested with or without 2.5% complement. Representative neutralization curves are shown (left). Error bars indicate standard deviations of technical replicates. Fold improvement of IC₅₀ values in the presence of 2.5% complement is indicated (right). Each dot represents the fold change between a pair of independent neutralization experiments, with or without complement. Improvements in IC₅₀ values are not significant for MACV (n = 5 independent experiments), but are significant for JUNV (n = 4 independent experiments, * indicates p < 0.05, Student’s two-tailed t test). b Arenacept activates complement. Cytotoxicity mediated by complement activation on HEK293T cells that transiently display the spike complex of JUNV (left) or of MACV (right). Cell killing was determined at Arenacept concentration of 10 μg/ml Arenacept-M1 or Arenacept-M3, as indicated. The difference in cytotoxicity between Arenacept-M1 and Arenacept-M3 in the case of JUNV is significant (n = 13 technical repeats, ** indicates p < 0.005, Student’s two-tailed t test). These are representative experiments out of three independent repeats. Boxes indicate standard deviations of technical repeats. Median and average values are indicated with horizontal lines and open circles, respectively. For all relevant panels, source data are provided as a source data file.