Fig. 1: Synthesis and pharmacological characterization of ParaMus.
From: Image-guided neural activity manipulation with a paramagnetic drug
a Chemical synthesis of ParaMus (7): (i) HOOC-PEG3-NHBoc, EDC/NHS, MOPS (100 mM, pH 5.5), (ii) TFA/DCM (80:20), (iii) DOTA-NHS, MOPS (25 mM, KCl 100 mM, pH 8), (iv) GdCl3, H2O, pH 5.5. b Setup for intracellular recording of primary rat cortical neurons (scale bar = 10 µm). c Representative current-clamp recordings in response to injection of 0.1-0.5 nA current injection in the presence of vehicle, 10 µM muscimol, 10 µM ParaMus, and 10 µM gadoteridol. Scale bars, horizontal = 20 ms, vertical = 10 mV. d Current thresholds for action potential generation before (−) and after (+) application of muscimol, ParaMus, or gadoteridol. Paired measurements are shown for two (muscimol), four (ParaMus), or three (gadoteridol) independent experiments.
