Fig. 4: Differences in levels of N acquisition transcripts in partially and fully populated SSB01.

Samples were taken 16 and 40 d after exposure of aposymbiotic anemones to algae (see Methods). a Fluorescence micrographs; scale bar, 2āmm. b flow-cytometer counts (normalized to protein amounts showing the difference in algal population densities between the 16-d and 40-d samples. c Relative abundances at 16 d and 40 d of algal transcripts encoding putative ammonium (AMT), nitrate (NRT), and adenine/guanine (NUP) transporters, and a putative nitrate reductase (NIT), as measured by RT-qPCR and expressed relative to the abundance of cyclophilin mRNA (see Methods and Fig.Ā 2). All RT-qPCR results are averages of three biological replicates and are expressed as meansā±āSDs; p-values (two-sided t-test) for the significance of the differences are shown (***p-valueā<ā0.001; **p-valueā<ā0.01). Source data are provided as a Source Data file.