Fig. 4: Differences in levels of N acquisition transcripts in partially and fully populated SSB01. | Nature Communications

Fig. 4: Differences in levels of N acquisition transcripts in partially and fully populated SSB01.

From: Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

Fig. 4: Differences in levels of N acquisition transcripts in partially and fully populated SSB01.The alternative text for this image may have been generated using AI.

Samples were taken 16 and 40 d after exposure of aposymbiotic anemones to algae (see Methods). a Fluorescence micrographs; scale bar, 2 mm. b flow-cytometer counts (normalized to protein amounts showing the difference in algal population densities between the 16-d and 40-d samples. c Relative abundances at 16 d and 40 d of algal transcripts encoding putative ammonium (AMT), nitrate (NRT), and adenine/guanine (NUP) transporters, and a putative nitrate reductase (NIT), as measured by RT-qPCR and expressed relative to the abundance of cyclophilin mRNA (see Methods and Fig.Ā 2). All RT-qPCR results are averages of three biological replicates and are expressed as means ± SDs; p-values (two-sided t-test) for the significance of the differences are shown (***p-value < 0.001; **p-value < 0.01). Source data are provided as a Source Data file.

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