Fig. 5: Photosynthetic function in SSB01 cells growing in hospite.
a–c Chlorophyll a levels and photosynthetic function were determined for algal cells at steady state in hospite (blue), in N-replete cultures (dark brown), and in cultures following N deprivation for 30 days (light brown), as described in Methods. a Chlorophyll a per cell. Means ± SDs for three independent trials are shown with the p-values (two-sided t-test) for the probabilities that the differences observed are significant (***p-value < 0.001). b Maximum PSII efficiencies Fv/Fm. Means ± SDs for six individual anemones and for four independent cultures are shown. (***p-value < 0.001; ns, p-value > 0.05). c PSI function assessed as the kinetics of P700 oxidation-reduction. White bar, 10 s of continuous nonsaturating illumination; arrowhead, time of saturating pulse. Blue, dark-brown, and light-brown arrows indicate total amounts of P700 oxidation (P700ox) determined for the cells in hospite, N-replete cultures, and N-depleted cultures, respectively. Mean values for four independent cultures and six sets of anemones are shown. d Volcano plot of relative abundances of transcripts encoding protein involved in photosynthesis. x-axis, fold-changes; y-axis, adjusted p-value based on Benjamini-Hochberg correction (Padj); red and blue, transcripts more abundant in hospite and in culture, respectively. Both axes use log scales. The horizontal line indicates adjusted p-value = 0.001, the cutoff used for considering differences to be significant (see text). Source data are provided as a Source Data file.
