Fig. 5: The HA-containing structures have not only lamellar membranous features but also electron-dense core features.
From: The inducible amphisome isolates viral hemagglutinin and defends against influenza A virus infection
a Correlative fluorescence and electron microscopy (EM) of MDCK cells stably expressing HAmVenus. The cells were infected with IAV strain PR8 at 10 MOI for 1 h. After washing, the cells were cultured in the presence of PVF-tet (20 μm) for 15 h. Arrowheads indicate the HA-containing structures; the arrow in the last panel indicates the lamellar membrane. b Electron microscopy of MDCK clone 6. The cells were not infected or infected with IAV strain PR8 at 2 MOI for 1 h. After washing, the cells were cultured for 15 h. Scale bars represent 6 μm (a, upper panel), 500 nm (a, lower panel), or 200 nm b, respectively.
