Fig. 1: Attenuation of senescence in est2∆ nup1-LexA cells.
From: The nuclear pore complex prevents sister chromatid recombination during replicative senescence
a Schematic structure of the nuclear basket protein Nup1 fused to LexA. b Mean senescence profiles of the est2∆ (n = 22) and est2∆ nup1-LexA (n = 28) clones analyzed in the course of this study. Each clone was isolated by sporulation of a heterozygous diploid strain. Each spore colony was then propagated in liquid culture through daily serial dilutions. OD600 was measured every day to estimate the cell density reached in 24 h. PD numbers were estimated from the initial spores. c Mean senescence profiles of est2∆ and est2∆ nup1-LexA clones in the absence of RAD52 (n = 4 and 8, respectively). Control est2∆ (n = 7) and est2∆ nup1-LexA (n = 5) curves are from the clones used in this specific experiment. d Mean senescence profiles of est2∆ and est2∆ nup1-LexA clones in the absence of RAD51 (n = 4 and 7, respectively). e Mean senescence profiles of est2∆ and est2∆ nup1-LexA clones in the absence of RAD59 (n = 4 and 6, respectively). Error bars indicate SD.
