Fig. 3: Nup1-LexA affects localization and stability of CAG trinucleotide repeats.
From: The nuclear pore complex prevents sister chromatid recombination during replicative senescence
a Yeast chromosome VI containing 130 integrated CAG repeats and a lacO array that binds the LacI-GFP protein. S-phase cells were imaged, and the location of the foci was scored into one of three zones of equal area, using the GFP-Nup49 signal to mark the nuclear periphery. b Percent of zone 1 foci for CAG-130 S-phase cells (283 cells for wild-type, 142 cells for nup1-LexA). Statistical differences were determined by a Fisher’s exact test (**p = 0.0012). c Frequency of contractions and expansions for CAG-70 repeats in wild-type and nup1-LexA cells. The frequency was determined by analysis of PCR amplicon length on a high-resolution fragment analyzer gel system, using PCR primers that flank the CAG-70 tract located on a YAC (see Fig. S6A); (***) p = 0.0001 compared with wild-type by Fisher’s exact test; (^) p = 0.0313; (^^) p = 0.0017 compared with nup1-LexA by Fisher’s exact test.
