Fig. 7: nup1∆Ct phenocopies the phenotypes of nup1-LexA.
From: The nuclear pore complex prevents sister chromatid recombination during replicative senescence
a Mean senescence profiles of est2∆ (n = 5) and est2∆ nup1∆FxFG (n = 5) clones. Error bars are SD. b Telomere length analysis of two representative est2∆ nup1∆FxFG clones. Southern blot of XhoI-digested DNA prepared from samples of senescing cells was revealed with a TG1–3 probe. c Mean senescence profiles of est2∆ (n = 4) and est2∆ nup1∆Ct (n = 8) clones. Error bars are SD. d Telomere length analysis of representative clones of the indicated genotypes. e The localization of the Cdc13-YFP/Rfa1-CFP foci in the three zones of equal area of the nucleus marked by Nic96-RFP was scored in late S and G2/M est2∆ and est2∆ nup1∆Ct cells as described in Fig. 2. The percentages of zone 1 foci are from three independently isolated est2∆ nup1∆Ct (n = 139 cells) clones. WT cells are from Fig. 3. Statistical differences were determined by a Fisher’s exact test (**p = 0.0019). f Percent of zone 1 foci for CAG-130 S-phase in nup1∆Ct cells (n = 143). Statistical differences were determined by a Fisher’s exact test (***p = 0,0006). WT and nup1-LexA cells are from Fig. 3. The repartition of the Rfa1/Cdc13 and CAG-130 foci between the three zones of the nucleus is shown in the Supplementary Fig. 8.
