Fig. 2: NBS1 regulates the nucleolar response to rDNA breaks.
From: Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation
a Timecourse of endogenous Treacle localization after I-Ppo1 transfection in normal U2OS cells and NBS1ΔN cells. b Quantification of nucleolar EU incorporation after I-Ppo1 H98A (n = 111) and I-Ppo1 WT (n = 87) transfection in control U2OS and after I-Ppo1 H98A (n = 116) and I-Ppo1 WT (n = 124) transfection in NBS1ΔN cells (boxes represent the 25–75 percentile range with median and whiskers represent the 5–95 percentile range. Data points outside of this range are shown individually). c Time-lapse microscopy of U2OS cells expressing mNG-tagged full-length NBS1 (wild type), after transfection with I-Ppo1. d Timeline of NBS1-mNG recruitment in relation to morphological changes in the nucleoli after I-Ppo1 transfection. e Confocal microscopy of NBS1-mNG wild type and point mutants in the NBS1ΔN background. f Localization of endogenous NBS1 2 h after I-Ppo1 transfection in siCtrl and siTreacle-treated cells (percentages of cells with >2 NBS1 caps are indicated; one of two experiments is shown). All scalebars = 10 µm. Source data are provided as a Source Data file.
