Fig. 5: Characterization of the TOPBP1–Treacle interaction.
From: Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation
a Schematic showing the layout of conserved domains and motifs in Treacle as well as HA-tagged deletion constructs of Treacle, lacking either the N-terminal region (F1), both the N-terminal and the central repeat region (F2), the central repeat region (F3), the C-terminal region (F4) or both the central repeat and the C-terminal region (F5). HA-immunoprecipitation of full-length and five deletion constructs depicted in a. c Schematic showing the layout of conserved domains and motifs in TOPBP1. Names of known TOPBP1-binding partners are shown below the domains they interact with and phospho-interacting Lys residues in the three BRCT tandems are highlighted. AAD: ATR-activating domain. d GFP-pulldowns from 293FT cells transfected with the indicated GFP-tagged TOPBP1 constructs. Source data are provided as a Source Data file.
