Fig. 7: Treacle- and TOPBP1-mediated nucleolar recruitment of ATR.
From: Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation
a ATR localization in Treacle- and TOPBP1-depleted U2OS cells and control cells 2 h after I-Ppo1 transfection. b Quantification of the experiment in a, siCtrl (n = 364), siTreacle (n = 214) and siTOPBP1 (n = 245) treated cells. Statistical significance was calculated using a one-way analysis of variance and Sidak’s multiple comparison test. Bars represent mean ± S.D. c ATR localization in 293-T cells overexpressing GFP-TOPBP1 wild type and W1145R AAD point mutant. d UBF localization in Hela cells overexpressing GFP-TOPBP1 and transfected with control siRNA and Treacle siRNA, respectively. All scale bars = 10 µm. Source data are provided as a Source Data file.
