Fig. 8: Loss of Treacle and TOPBP1 impairs rDNA repair.
From: Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation
a γH2AX, 53BP1, RPA2, RAD51 and BRCA1 localization in control depleted (siCtrl) and Treacle-depleted (siTreacle) U2OS cells 2 h after I-Ppo1 transfection. b Quantification of the experiment in a, γH2AX: siCtrl (n = 177) versus siTreacle (n = 200), 53BP1: siCtrl (n = 261) versus siTreacle (n = 239), RPA2: siCtrl (n = 152) versus siTreacle (n = 168) BRCA1: siCtrl (n = 525) versus siTreacle (n = 246) and RAD51: siCtrl (n = 525) versus siTreacle (n = 246) cells (all datapoints are shown, bar represents mean, error bars represent S.D.) c γH2AX, 53BP1, RPA2, RAD51 and BRCA1 localization in control depleted (siCtrl) and TOPBP1-depleted (siTOPBP1) U2OS cells 2 h after I-Ppo1 transfection. d Quantification of the experiment in c, γH2AX: siCtrl (n = 403) versus siTOPBP1 (n = 205), 53BP1: siCtrl (n = 239) versus siTOPBP1 (n = 256), RPA2: siCtrl (n = 649) versus siTOPBP1 (n = 359) BRCA1: siCtrl (n = 228) versus siTOPBP1 (n = 348) and RAD51: siCtrl (n = 228) versus siTOPBP1 (n = 348) cells (all datapoints are shown, bar represents mean, error bars represent S.D.). e Clonogenic surivival analysis of I-Ppo1-transfected control (siCtrl), Treacle-depleted (siTreacle) and TOPBP1-depleted (siTOPBP) U2OS cells (red bars represent the mean). f Clonogenic surivival analysis of I-Ppo1-transfected WT and NBS1DN U2OS cells (red bars represent the mean). g Model (see text for details). Source data are provided as a Source Data file.
