Table 2
From: Modeling medulloblastoma in vivo and with human cerebellar organoids
Primary antibodies | Host species | Dilution | Company | Reference |
|---|---|---|---|---|
BARHL1 | Rabbit | 1:1000 (organoids) 1:500 (tissues) | Atlas Antibodies | HPA004809 |
β3-tubulin | Mouse | 1:2000 | Thermofisher Scientific | MA1-118 |
Calbindin-D-28K | Rabbit | 1:500 | Sigma Aldrich | C9848 |
Cleaved Caspase-3 (D175) | Rabbit | 1:200 | Cell Signaling Technology | 9661 |
c-MYC (Y69) | Rabbit | 1:200 | Abcam | ab32072 |
FLAG M2 | Mouse | 1:100 | Sigma Aldrich | F1804 |
GFAP | Rabbit | 1:200 | Sigma Aldrich | G9269 |
GFP | Chicken | 1:2000 | Abcam | ab13970 |
GFP | Rabbit | 1:500 | Thermofisher Scientific | A11122 |
Ki67 | Rabbit | 1:500 | Abcam | ab15580 |
NeuN | Rabbit | 1:2000 | EMD Millipore Corporation | ABN78 |
NPR3 | Rabbit | 1:100 | Abcam | ab37617 |
OLIG2 | Rabbit | 1:200 | Abcam | ab33427 |
OTX2 | Mouse | 1:100 | Santa Cruz Biotechnology | sc-514195 |
PCNA | Mouse | 1:2000 (organoids) 1:500 (tissues) | EMD Millipore Corporation | MAB424 |
PH3 | Rat | 1:500 | Abcam | ab10543 |
SMARCA4/BRG1 (G-7) | Mouse | 1:100 | Santa Cruz Biotechnology | sc-17796 |
SKOR2 | Rabbit | 1:1000 | Atlas Antibodies | HPA046206 |
SOX2 | Rabbit | 1:1000 (organoids) 1:500 (tissues) | Abcam | ab97959 |
SOX9 | Rabbit | 1:4000 (organoids) 1:2000 (tissues) | EMD Millipore Corporation | AB5535 |
Secondary antibodies | Dilution | Company | Reference |
|---|---|---|---|
Alexa Fluor 488 goat anti-chicken IgY | 1:500 | Thermofisher Scientific | A11039 |
Alexa Fluor 488 goat anti-rabbit IgG | 1:500 | Thermofisher Scientific | A11008 |
Alexa Fluor 546 goat anti-mouse IgG | 1:500 | Thermofisher Scientific | A11030 |
Alexa Fluor 647 goat anti-mouse IgG | 1:500 | Thermofisher Scientific | A21235 |
Alexa Fluor 546 goat anti-rabbit IgG | 1:500 | Thermofisher Scientific | A11035 A11010 |
Alexa Fluor 647 goat anti-rabbit IgG | 1:500 | Thermofisher Scientific | A21245 |
Alexa Fluor 647 goat anti-rat IgG | 1:500 | Thermofisher Scientific | A21247 |
Goat anti-Rabbit IgG-heavy and light chain Biotinylated | 1:250 | Bethyl Laboratories Inc. | A120-101B |
Goat anti-Mouse IgG-heavy and light chain Biotinylated | 1:250 | Bethyl Laboratories Inc. | A90-116B |
