Fig. 1: Glucose intolerance in Irp2^−/− mice is not caused by impaired peripheral and hepatic insulin action.

a Intraperitoneal glucose tolerance tests (ipGTTs) for 5-month-old WT and Irp2^−/− mice (2 g glucose/kg body weight). b Glucose AUC calculated from ipGTTs for WT and Irp2^−/− mice at the indicated ages. c Plasma glucose concentrations for fasted WT and Irp2^−/− mice. d Insulin tolerance tests (ITTs) performed in random-fed 5-month-old WT and Irp2^−/− mice. Glucose AUC graph is shown on the right. e–g Euglycemic-hyperinsulinemic clamp experiments conducted in fasted 7-month-old WT and Irp2^−/− mice. Glucose infusion rate (e), whole-body glucose turnover rate (f), and hepatic glucose production (g). Data in a–g are expressed as means ± s.e.m., unpaired two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001 relative to WT mice. The number of mice is indicated in parentheses. Source data are provided as a Source Data file.