Fig. 2: Leakage of chromatin-proximal lysosomes during mitosis in vivo.
From: Spatially and temporally defined lysosomal leakage facilitates mitotic chromosome segregation
a Representative confocal images of chromatin-proximal leaky lysosomes in murine intestinal crypts stained for LGALS3, mitotic chromatin (P-S10-H3) and DNA. b Quantification of (a). Dot plots, mean ± SD, n = 3 mice with ≥324 mitotic and ≥356 interphase cells from 3 non-consecutive sections analyzed for each mouse. See Supplementary Fig. 2a for the co-localization of LGALS3 and LAMP1 in intestinal crypts. c Representative confocal images of chromatin-proximal leaky lysosomes in orthotopic MCF7 breast carcinoma xenografts from Balb/c nude mice stained for LGALS3, LAMP1, and DNA. (d) Quantification of (c). Dot plots, mean ± SD, n = 4 mice with ≥165 mitotic and ≥156 interphase cells from 3 non-consecutive sections analyzed for each mouse. Arrows, chromatin-proximal leaky lysosomes. P-values were calculated by unpaired, two-tailed Students t-test. Scale bars, 10 µm. Source data for b, d are provided as Source Data files.
