Fig. 3: S. pombe Mre11/Rad50 and E. coli Mre11/Rad50 (SbcCD) are structurally similar to human Mre11/Rad50.
From: Rad50 zinc hook functions as a constitutive dimerization module interchangeable with SMC hinge
a (i) ring and (ii) open/arm-connected structures of S. pombe Mre11/Rad50 (−ATP). “H”: the hook. b Sequential images showing movement of coiled-coil arms of S. pombe Mre11/Rad50. Imaging speed: 500 msec per frame. c Various structures observed with E. coli SbcCD (−ATP). (i) open/arm-connected, (ii) head-open, (iii) ring, and (iv) “S-shaped” structures. d SbcCD was real-time imaged by adding the protein to the imaging chamber while scanning (−ATP). The structural transients are shown. Imaging speed: 150 msec per frame. Scan size, 100 × 100 nm. Representative images selected from the movie of a single SbcCD molecule (Supplementary Movie 7) are shown with schematic diagrams of presumed structures.
