Fig. 5: KDM6s are essential in the epigenetic switch from NPCs to neural subtypes.
From: JMJD3 and UTX determine fidelity and lineage specification of human neural progenitor cells
a Heatmap of upregulated or downregulated genes in wild-type (WT)-D28 compared with wild-type (WT) NPCs. Expression levels are shown as fold changes (log2) compared with expression levels in WT NPCs. b, c Top 15 GO terms most enriched in up- or downregulated genes in differentiated WT-D28 cells. d Left panel, heatmap of ATAC-seq data indicating regions with differential chromatin accessibility between WT NPCs and differentiated cells at D28. Right panel, numbers of OC or CO regions. OC, open-to-closed, indicates regions with reduced chromatin accessibility in differentiated cells at D28. CO, closed-to-open, indicates regions with increased chromatin accessibility in differentiated cells at D28. e GO analysis for OC region-associated genes described in d. f ATAC-seq analysis indicating genes downregulated in differentiated WT-D28 cells compared with WT NPCs. g Heatmap and GO analysis of differentially expressed genes between WT and dKO cells at day 28 of differentiation. dKO, deletion of both JMJD3 and UTX. h Signal densities from ATAC-seq data for the indicated regions described in d. i Genomic views of genes involved in neuronal development indicated by ATAC-seq data from differentiated WT-D28 or dKO-D28 cells. WT wild type.
