Fig. 6: NMVs contain miRNAs that are delivered to HCAEC and alter gene expression.

a miRNA content of NMVs prepared from stimulated human neutrophils quantified by RT-qPCR (n = 5). b miR-155 and miR-223 content of MVs in human plasma pre- and post high-fat diet (HFD, n = 5), or (c) plasma from mice fed chow or Western diet (WD, 6 weeks; n = 5) was quantified by RT-qPCR. d miR-155 expression levels in NMVs isolated from mice fed chow or Western diet (n = 3) was measured by RT-qPCR. e HCAEC were incubated with (+NMV) or without (−NMV) NMVs for 2 h and miR-155 (n = 10) and BCL6 (n = 9) expression levels measured by RT-qPCR. f HCAEC were incubated with NMVs prepared from human neutrophils isolated pre- and post HFD and miR-155 expression levels quantified by RT-qPCR (n = 3). The dotted line shows the mean copy number per HCAEC in the absence of NMVs. g HCAEC were incubated with NMVs prepared from miR-155^−/− vs. wild type mouse neutrophils and miR-155 expression levels quantified by RT-qPCR (n = 3). h HCAEC were transfected with 25 ρmol of miR-155 antagomir (A) or scrambled control (Sc) and incubated with NMVs for 2 h. HCAEC expression of BCL6, RELA and its downstream targets was investigated by RT-qPCR (n = 5). Data are presented as mean ± SEM and statistical significance evaluated using a paired or unpaired t-test as appropriate. NS not statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. All n numbers represent independent experiments. Source data are provided as a Source Data file.