Fig. 2: Arf1 ablation induces the infiltration and activation of immune cells in Lgr5/Apc mice.

a, b Immunofluorescent staining of GFP and CD8a (a) or CD4 (b) in the intestine of the indicated mice. c–e Flow cytometric analysis of gut APCs: DCs (c), inflammatory DCs (d), and macrophages (e) (n = 3 mice each group; **p < 0.01, t-test; repeat two independent experiments). f–m Flow cytometric analysis of the immune cells of LPLs: CD4⁺ T cells (f), IFNγ⁺CD4⁺ T cells (g), CD8⁺ T cells (h), IFNγ⁺CD8⁺ T cells (i), γδ T cells (j), NK cells (k), Treg cells (l), and IL-13⁺ CD4⁺ T cells, (m) (n = 3 or 5). n–p Flow cytometric analysis of the immune cells of IELs: CD4⁺ T cells (n), CD8αβ⁺ T cells (o), and CD8αα⁺ T cells (p) (n = 5 mice per group; *p < 0.05, **p < 0.01, t-test; repeat two independent experiments). q–u Relative gene expression of the indicated chemokines, cytokines, and granzymes (n = 5 mice each group, repeat two independent experiments). v Immunofluorescent staining of GFP and pZap70 in the intestine of the indicated mice (n = 5 mice each group; *p < 0.05, **p < 0.01, ***p < 0.001, t-test; repeat two independent experiments). Each experiment replicate two times. Data are shown as the mean ±  SEM. Scale bars are as indicated.